• Login
    View Item 
    •   Mak IR Home
    • College of Health Sciences (CHS)
    • Infectious Diseases Institute (IDI)
    • Infectious Diseases Institute (IDI) Collections
    • View Item
    •   Mak IR Home
    • College of Health Sciences (CHS)
    • Infectious Diseases Institute (IDI)
    • Infectious Diseases Institute (IDI) Collections
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    New manual quantitative polymerase chain reaction assay validated on tongue swabs collected and processed in Uganda shows sensitivity that rivals sputum-based molecular tuberculosis diagnostics

    Thumbnail
    View/Open
    Research article (631.4Kb)
    Date
    2024
    Author
    Steadman, Amy
    Andama, Alfred
    Ball, Alexey
    Mukwatamundu, Job
    Khimani, Khushboo
    Mochizuki, Tessa
    Asege, Lucy
    Bukirwa, Alice
    Kato, John Baptist
    Katumba, David
    Kisakye, Esther
    Mangeni, Wilson
    Mwebe, Sandra
    Nakaye, Martha
    Nassuna, Irene
    Nyawere, Justine
    Nakaweesa, Annet
    Cook, Catherine
    Phillips, Patrick
    Nalugwa, Talemwa
    Bachman, Christine M
    Semitala, Fred Collins
    Weigl, Bernhard H
    Connelly, John
    Worodria, William
    Cattamanchi, Adithya
    Metadata
    Show full item record
    Abstract
    Background: Sputum-based testing is a barrier to increasing access to molecular diagnostics for tuberculosis (TB). Many people with TB are unable to produce sputum, and sputum processing increases assay complexity and cost. Tongue swabs are emerging as an alternative to sputum, but performance limits are uncertain. Methods: From June 2022 to July 2023, we enrolled 397 consecutive adults with cough >2 weeks at 2 health centers in Kampala, Uganda. We collected demographic and clinical information, sputum for TB testing (Xpert MTB/RIF Ultra and 2 liquid cultures), and tongue swabs for same-day quantitative polymerase chain reaction (qPCR) testing. We evaluated tongue swab qPCR diagnostic accuracy versus sputum TB test results, quantified TB targets per swab, assessed the impact of serial swabbing, and compared 2 swab types (Copan FLOQSWAB and Steripack spun polyester). Results: Among 397 participants, 43.1% were female, median age was 33 years, 23.5% were diagnosed with human immunodeficiency virus, and 32.0% had confirmed TB. Sputum Xpert Ultra and tongue swab qPCR results were concordant for 98.2% (95% confidence interval [CI]: 96.2-99.1) of participants. Tongue swab qPCR sensitivity was 92.6% (95% CI: 86.5 to 96.0) and specificity was 99.1% (95% CI: 96.9 to 99.8) versus microbiological reference standard. A single tongue swab recovered a 7-log range of TB copies, with a decreasing recovery trend among 4 serial swabs. Swab types performed equivalently. Conclusions: Tongue swabs are a promising alternative to sputum for molecular diagnosis of TB, with sensitivity approaching sputum-based molecular tests. Our results provide valuable insights for developing successful tongue swab-based TB diagnostics.
    URI
    10.1093/cid/ciae041
    http://hdl.handle.net/10570/14657
    Collections
    • Infectious Diseases Institute (IDI) Collections

    DSpace 5.8 copyright © Makerere University 
    Contact Us | Send Feedback
    Theme by 
    Atmire NV
     

     

    Browse

    All of Mak IRCommunities & CollectionsTitlesAuthorsBy AdvisorBy Issue DateSubjectsBy TypeThis CollectionTitlesAuthorsBy AdvisorBy Issue DateSubjectsBy Type

    My Account

    LoginRegister

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    DSpace 5.8 copyright © Makerere University 
    Contact Us | Send Feedback
    Theme by 
    Atmire NV