| dc.description.abstract | Back ground: In Uganda, 12% to 25% of the population is living with peptic ulcer disease. Efforts to treat the disease have become much more difficult due to rising prevalence of drug resistance, unbearable adverse drug reactions and high costs of purchasing conventional medicines. This has made them un accessible to the less economically privileged people hence creating need to search for and develop new inexpensive and effective anti-ulcer medicines to help in combatting the situation.
Objectives: The aims of the study were to determine the percentage gastroprotective ratio (PGPR), percentage gastrocurative ratio (PGCR), its effect on gastric mucus concentration and nitric oxide levels, volume of secreted gastric juice, its total acidity and the histopathological effect of fresh S. officinarum juice extract (SOJE) goa (var) against aspirin induced gastric ulcers.
Methods: The study was experimental, and laboratory based which involved studying two models i.e., gastroprotective and gastrocurative ulcer treatment models. Ulcers were induced in wistar albino rats using one oral dose of 300mg/kg aspirin. SOJE was given at doses of 0.75, 1.5, and 2.25ml/100gbwt. Refined sugar was given at 250mg/kg bwt, Misoprostal (100µg/kg) and esomeprazole (40mg/kg) were the reference drugs for the respective models. SOJE treatment was given for 14 days after which blood samples, gastric juice and stomachs of sacrificed rats were collected and taken for determination of mucus and Nitric oxide concentration, gastric juice volume, its total acidity, ulcer score and ulcer indices and histopathology.
Results: In the gastroprotective model, treatment with SOJE at a dose of 0.75ml/100g exhibited a PGPR of 45%, 1.5ml/100g bwt had PGPR of 55% and 2.25ml/100g had PGPR of 64%. Table sugar group exhibited the lowest PGPR of 38%. In the gastrocurative model, SOJE at a dose of 0.75ml/100g had PGCR of 66%, 1.5ml/100g had PGCR of 72% and 2.25ml/100g had PGCR of 72% and table sugar had a PGCR of 61%. SOJE exhibited potential to lower blood nitric oxide levels, gastric juice volume and its total acidity and increased stomach mucus deposition. SOJE also significantly attenuates necrosis of the stomach mucosal wall in the gastrocurative and gastroprotective models.
Conclusion: The results suggest that the fresh SOJE, at a dose between 1.5- 2.25ml/100g body weight, shows gastroprotective and gastrocurative potential against aspirin-induced gastric ulceration via increased mucus deposition, reduction of volume of digestive juice secreted and total acidity. | en_US |
