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dc.contributor.authorSebulime, Sam Andrew
dc.date.accessioned2024-12-18T09:26:37Z
dc.date.available2024-12-18T09:26:37Z
dc.date.issued2024-11-18
dc.identifier.citationSebulime, S.A. (2024). Cytokine profiles of HIV-associated COPD in a Ugandan cohort. (Unpublished masters dissertation) Makerere University, Kampala, Uganda.en_US
dc.identifier.urihttp://hdl.handle.net/10570/14216
dc.descriptionA dissertation submitted to the directorate of research and graduate training in partial fullfilment of the requirements for the award of the degree of masters of science in immunology and clinical microbiology of makerere University.en_US
dc.description.abstractIntroduction:COPD is a chronic lung condition characterized by irreversible airflow obstruction and encompasses chronic bronchiolitis and emphysema. Cigarette smoking is the predisposing factor in over 50% of cases but more risk factors are increasingly being discovered. These include Biomass gas exposure, air pollution, HIV/AIDS, TB, pesticide exposure, etc. While much is known about the pathogenesis of COPD, a great majority of this data has been generated based on Cigarette smoke induced models of COPD. Thus, this study seeks to investigate the cytokine profiles underlying HIV-associated COPD. Objectives: 1) To profile the cytokines driving inflammation in a rural cohort of COPD patients compared with negative controls. 2) To compare and contract cytokine levels in plasma and sputum of the different study sub-groups (HIV-COPD-, HIV-COPD+, HIV+COPD-, and HIV+COPD+). Methods: Induced sputum and plasma samples from 8 HIV-COPD-, 11 HIV-COPD+, 9HIV+COPD-, & 10 HIV+COPD+ were retrieved, thawed and analyzed using a 48panel Bio-plex pro human assay kit (Bio-Rad Inc). ODs obtained were analyzed using GraphPad Prism 10 and R-studio. Heat map analysis and Box plots were used to compare cytokine levels between groups. Differences in cytokine levels reaching statistical significance (p-value< 0.05) were considered in the analysis.RESULTS: We saw elevation in levels of some but not all pro-inflammatory cytokines in plasma of COPD+ clients compared to COPD-. There was elevation in both Th1 and Th2 cytokines in sputum of HIV+COPD-, HIV-COPD+, and HIV+COPD+ compared to double negative controls. Plasma had generally lower levels of all pro-inflammatory cytokines.CONCLUSION: Our study has demonstrated elevation in cytokine levels of some but not all pro-inflammatory cytokines, particularly, IL-8, IL-1Ra, MIG, RANTES, MIF, and IP-10 in plasma of COPD+ versus COPD negative samples. Sub-group analysis produced mixed results, with elevations of particular cytokines such as IL-4, IL-12p40, PDGF-BB and IL-7 in sputum, but generally lower cytokine levels in plasma compared to negative controls.en_US
dc.description.sponsorshipMakerere Lung Institute Makerere MITHU Granten_US
dc.language.isoenen_US
dc.publisherMakerere Universityen_US
dc.subjectCytokineen_US
dc.subjectCOPDen_US
dc.subjectHIVen_US
dc.subjectUgandaen_US
dc.titleCytokine profiles of HIV-associated COPD in a Ugandan Cohorten_US
dc.typeOtheren_US


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